Title: [115] Characterization of phosphorus compounds by acid lability
Abstract: Measurements of the rate of hydrolysis of phosphoric esters have been carried out for analyzing mixtures, as a test of homogeneity, and as a criterion of identity. This chapter presents the procedure for the estimation of phosphate. Of the many methods available for the estimation of phosphate, that of Fiske and SubbaRow is one of the simplest and most widely used. In the Fiske and SubbaRow procedure, the extra-labile compounds are estimated as inorganic phosphate because of the relatively high acid concentration (pH 0.65) and because molybdate accelerates the hydrolysis of some organic phosphates. By measuring the color immediately after adding the reagents, however, Fiske and SubbaRow were able to estimate phosphocreatine. Better results are obtained by estimating the true inorganic phosphate by precipitating it with magnesium mixture or with calcium salts and ethanol. Other method is the Lowry and Lopez method in which the acid and molybdate concentrations are lower than in Fiske and Subba-Row's, so that some extra-labile compounds are hydrolyzed more slowly.
Publication Year: 1957
Publication Date: 1957-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
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Cited By Count: 266
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