Title: [9] The kinetics of immobilized enzyme systems
Abstract: This chapter reviews that most kinetic studies on enzyme-catalyzed reactions are made with both the enzyme and the substrate in free solution. It discusses the kinetic principles that apply to immobilized enzymes and some of the applications of these principles to the systems that have been investigated experimentally. There are several reasons to investigate the kinetics of immobilized enzymes. In the first place, enzymes in nature are frequently immobilized in cells or tissues and kinetic studies in free solution, although providing an important basis for understanding do not give the entire answer, as to how the enzymes function in the living organism. Second, chemical engineers are becoming increasingly interested in the use of enzymes as catalysts for industrial processes. Third, immobilized enzymes lead to important procedures in clinical medicine. Another important clinical application is the automated analysis of important metabolities, biological fluid being pumped into one end of a tube and an analysis made of the effluent. The chapter explains the kinetic behavior observed when an enzyme is included within a solid matrix that is in contact with a solution, containing the substrate and, also, with the situation, in which the enzyme is attached to the interior surface of a tube, through which substrate solution is flowing.
Publication Year: 1980
Publication Date: 1980-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 96
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