Title: Double-stranded RNA elements and their association with pathogenicity of Sclerotinia sclerotiorum
Abstract: Double-stranded RNA(dsRNA) elements and their pathogenicity of 16 strains of Sclerotinia sclerotiorum collected from eggplant grown in Jiamusi of Heilongjiang province,China,were investigated.Transmission of the abnormal hyphal-growth characteristics including slowly-growing and abnormal branchings from 5 hypovirulent strains of S.sclerotiorum to virulent strains of the pathogen were determined on agar medium.The results showed that pathogenicity of these strains of S.sclerotiorum on detached leaves of oilseed rape(Brassica napus) differed greatly.Three pathogenicity types were classified with 7 strains belonging to the strong pathogenicity type(SPT),7 belonging to the weak pathogenicity type(WPT) and the other 2 belonging to the intermediate pathogenicity type(IPT).dsRNA elements were detected from the mycelia of 10 strains of S.sclerotiorum.Three SPT strains contained 7.4 kb dsRNA,six WPT strains contained both 6.4 kb and 7.4 kb dsRNA and one WPT strain contained only 6.4 kb dsRNA.But no dsRNA was detected in 4 SPT strains and 2 IPT strains of S.sclerotiorum.Therefore,the 6.4 kb dsRNA was more closely related to virulence atte-nuation(hypovirulence) of S.sclerotiorum than the 7.4 kb dsRNA.The results also indicated that the abnormal hyphal-growth characteristics of the five hypovirulent strains of S.sclerotiorum could be transmitted to vi-rulent strains of this fungus in a strain-specific manner.This study further suggests the importance of the 6.4 kb dsRNA in association with hypovirulence of S.sclerotiorum.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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Cited By Count: 2
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