Title: Cyclin is a component of the sea urchin egg M-phase specific histone H1 kinase.
Abstract: Research Article1 August 1989free access Cyclin is a component of the sea urchin egg M-phase specific histone H1 kinase. L. Meijer L. Meijer CNRS, Station Biologique, Roscoff, France. Search for more papers by this author D. Arion D. Arion CNRS, Station Biologique, Roscoff, France. Search for more papers by this author R. Golsteyn R. Golsteyn CNRS, Station Biologique, Roscoff, France. Search for more papers by this author J. Pines J. Pines CNRS, Station Biologique, Roscoff, France. Search for more papers by this author L. Brizuela L. Brizuela CNRS, Station Biologique, Roscoff, France. Search for more papers by this author T. Hunt T. Hunt CNRS, Station Biologique, Roscoff, France. Search for more papers by this author D. Beach D. Beach CNRS, Station Biologique, Roscoff, France. Search for more papers by this author L. Meijer L. Meijer CNRS, Station Biologique, Roscoff, France. Search for more papers by this author D. Arion D. Arion CNRS, Station Biologique, Roscoff, France. Search for more papers by this author R. Golsteyn R. Golsteyn CNRS, Station Biologique, Roscoff, France. Search for more papers by this author J. Pines J. Pines CNRS, Station Biologique, Roscoff, France. Search for more papers by this author L. Brizuela L. Brizuela CNRS, Station Biologique, Roscoff, France. Search for more papers by this author T. Hunt T. Hunt CNRS, Station Biologique, Roscoff, France. Search for more papers by this author D. Beach D. Beach CNRS, Station Biologique, Roscoff, France. Search for more papers by this author Author Information L. Meijer1, D. Arion1, R. Golsteyn1, J. Pines1, L. Brizuela1, T. Hunt1 and D. Beach1 1CNRS, Station Biologique, Roscoff, France. The EMBO Journal (1989)8:2275-2282https://doi.org/10.1002/j.1460-2075.1989.tb08353.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info A so-called ‘growth-associated’ or ‘M-phase specific’ histone H1 kinase (H1K) has been described in a wide variety of eukaryotic cell types; p34cdc2 has previously been shown to be a catalytic subunit of this protein kinase. In fertilized sea urchin eggs the activity of H1K oscillates during the cell division cycle and there is a striking temporal correlation between H1K activation and the accumulation of a phosphorylated form of cyclin. H1K activity declines in parallel with proteolytic cyclin destruction of the end of the first cell cycle. By virtue of the high affinity of the fission yeast p13suc1 for the p34cdc2 protein, H1K strongly binds to p13-Sepharose beads. Cyclin, p34cdc2 and H1K co-purify on this affinity reagent as well as through several conventional chromatographic procedures. Anticyclin antibodies immunoprecipitate the M-phase specific H1K in crude extracts or in purified fractions. Sea urchin eggs appear to contain much less cyclin than p34cdc2, suggesting that p34cdc2 may interact with other proteins. These results demonstrate that cyclin and p34cdc2 are major components of the M-phase specific H1K. Previous ArticleNext Article Volume 8Issue 81 August 1989In this issue RelatedDetailsLoading ...