Title: Screening and identification of a bacterial strain with antagonistic activity to wheat Fusarium graminearum.
Abstract: 【Objective】The present studies were performed to isolate microbial strain with antagonistic activity to wheat Fusarium graminearum, and explore the biological control of wheat scab. 【Method】The dilution spread plate method and the plate confrontation method were used to screen the antagonistic strains from 24 soil samples planted wheat with F. graminearum disease previously. 【Result】There were 65 strains isolated from the samples and 8 stains were found to be antagonistic towards F. graminearum. Of the 8 isolates, strain Jc-07 showed the strongest inhibition effect. Based on the morphological, physiological and biochemical characteristics as well as the sequencing of 16S rDNA, the strain Jc-07 was preliminary identified as Bacillus amyloliquefaciens. Fungistatic activity assay result indicated that the culture filtrate of strain Jc-07 was effective in inhibiting the mycelium growth of F. graminearum,and significantly reduced the spore germination rate. 【Conclusion】The strain Jc-07 displayed relatively high inhibition activity to Fusarium graminearum and had the potential in biological control of wheat scab.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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Cited By Count: 6
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