Title: Genetic Diversity of Elymus sibiricus Populations from the Northwestern Plateau of Sichuan by RAPD Markers
Abstract:Random amplified polymorphic DNA (RAPD) markers were used to assess the genetic diversity and population structure of eight natural Elymus sibiricus L. populations from the northwestern plateau of Sic...Random amplified polymorphic DNA (RAPD) markers were used to assess the genetic diversity and population structure of eight natural Elymus sibiricus L. populations from the northwestern plateau of Sichuan, a part of Qinghai-Tibet Plateau zone. Of the 150 primers screened, 25 produced highly reproducible RAPD bands. Using this 25 primers, 370 discernible DNA fragments were generated with 291 (78.65%) being polymorphic, indicating that considerable genetic variation was high at the species level. In contrast, there were relatively low levels of polymorphism at the population level with the percentage of polymorphic loci (PP) ranging from 46.49% to 53.78%. The mean Nei’s gene diversity (HE) was estimated to be 0.176 within populations (range 0.159 to 0.190), and 0.264 at the species level. The degree of genetic differentiation among the populations was estimated by Nei's genetic diversity (GST), Bayesian methods (GST-B) and Shannon’s indexes. The GST and GST-B values were 0.320 and 0.335, respectively. When using Shan-non’s index of phenetic diversity to quantify and further partition the amount of genetic diversity, 33.7% of the total genetic diversity was found among the populations. Analysis of molecular variance (AMOVA) revealed that genetic diversity was greater within populations (59.9%, P 0.001) than among populations (40.1%, P 0.001). The average exchanged number of individuals between populations per generation (Nm) was 0.503. A geographical pattern of population differentiation was revealed by both the cluster and AMO-VA analyses. Results showed that the E. sibiricus populations from south and north of sampling sites were clearly separated from each other, and high molecular variation occurred within populations from southeast of Qinghai-Tibet Plateau. In-situ conservation should be carried out on populations with higher genetic diversity.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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Cited By Count: 1
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