Title: Effect of a nuclepolyhedrovirus of<i>Autographa californica</i>expressing the enhancin gene of<i>Trichoplusia ni</i>granulovirus on<i>T. ni</i>larvae
Abstract: Abstract A recombinant Autographa californica nucleopolyhedrovirus (AcMNPV) strain showing higher virulence against Trichoplusia ni larvae than the wild-type virus was developed. The 'enhancin' (VEF) gene of T. ni granulovirus (TnGV) and the AcMNPV polyhedrin gene were cloned into the baculovirus transfer vector pAcUW31. This plasmid and AcMNPV BacPAK6 DNA were co-transfected into the BTI-Tn5B1-4 cell line. A recombinant AcMNPV strain (BacVEFPol) was purified, amplified, and bioassayed against T. ni first instar larvae. Its estimated LC50 (0.184 OB/mm2) was 2.18 times lower than the LC50 estimated for the wild-type AcMNPV (0.402 OB/mm2). Likewise, an LT50 of 67.7 h was estimated for the recombinant AcMNPV strain while the LT50 of wild-type AcMNPV was estimated at 81.9 h. This indicates a 17.4% reduction of the time required to kill the larvae. The higher virulence of the recombinant strain, evidenced by its LC50 and LT50 values being lower than those of the wild-type strain, indicates that the VEF protein is expressed properly and may be occluded in the OBs. Keywords: EnhancinpolyhedrinVEFAcMNPVTnGVTrichoplusia ni Published online 20 July 2005 Published online 20 July 2005 Acknowledgments We are indebted to Robert R. Granados, Brian A. Federici and James E. Throne for providing essential material. Authors wish to thank the excellent technical support of Javier Luévano and Laura Aguilar. This work was partially supported by grant 26312-N by CONACYT, Mexico. Notes Published online 20 July 2005
Publication Year: 2005
Publication Date: 2005-11-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 12
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