Title: [13] The isolation and characterization of pseudouridine
Abstract: The chapter discusses the principles, materials, and the procedure followed in the preparation of pseudouridylate and pseudouridine. Pseudouridine (5-β-D-ribosyluracil) is a constituent nucleoside of RNA, particularly in those RNA's known collectively as “sRNA” and “tRNA.” Pseudouridine appears as the free nucleoside in mammalian urine (man, rat, mouse) because of tRNA degradation. The most characteristic property of pseudouridine and its derivatives is that it allows detection, even in the presence of its usual contaminant, uridine, is the bathochromic shift in the ultraviolet-absorbance spectrum in alkaline media. To prepare pseudouridine from pseudouridylate, pseudouridylate is dephosphorylated and recovered by standard procedures from the enzyme solution.. Separations of pseudouridine from uridine, of pseudouridylate from uridylate, and of isomeric forms of pseudouridine itself, can be effected on paper in various solvent systems. The most useful and rapid one is isobutyrate buffer, in which the pseudo compounds have distinctly lower RI values than their uridine analogs. This is possibly the best way to separate uridine and pseudouridine in small quantities. Isopropanolacetic acid, butanol-H20, and so on, are employed as well for analytical purposes.
Publication Year: 1967
Publication Date: 1967-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
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Cited By Count: 4
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