Title: An Analytical Survey of a Biotinylated Bacteriophage System for Quantifying Transduction Events in Natural Ecosystems
Abstract: ! Bacteriophage transduction events play a contributive role in bacterial gene diffusion in ecosystems comprised of natural microbial populations. The rate at which these phage-mediated genetic transfers occur in wastewater ecosystems is not well understood. This work describes the effectiveness of a genetically engineered biotinylated bacteriophage T4 system as a tool for examining transduction event rates and compositions in environments that pose increased risk of antibiotic resistance proliferation. ! Antibiotic use has steadily increased over the past century, giving rise to antibiotic resistance in microorganisms. Agricultural, medical, and industrial use of antibiotics produces waste teeming with residual antibiotic compounds that are collected to form novel microbial ecosystems. These wastewater microbial communities include, among others, bacteria and their viral parasites, bacteriophage. Bacteriophage can transfer genetic material between bacterial hosts through a phenomenon known as transduction, thus creating circumstances that can contribute to the spread of antibiotic resistance genes. Wastewater ecosystems that contain vestigial antibiotic compounds may produce selective breeding grounds for the development of antibiotic resistant microorganisms. This study aimed to elucidate the role of bacteriophage in the spread of antibiotic resistant genes under these environmental conditions using a novel biotinbased tagging and recovery system. This system analyzed the unbiased tagging of bacteriophage particles, infection of host bacteria, and the recovery of biotinylated phage progeny as means to investigate phage-mediated transduction rates.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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