Title: [39] Inhibition of the dolichol pathway of protein glycosylation
Abstract: This chapter indicates how to inhibit glycosylation in vivo and in vitro, discusses the properties of the various inhibitors, and illustrates how to check whether inhibition of glycosylation is really achieved under the chosen experimental conditions. The table presented in the chapter indicates the sites of action of various inhibitors of glycosylation, the concentration range recommended, and the cell type in which they have been used. Protocols for the use of 2-deoxy-D-glucose (2dGlc), 2-deoxy-2-fluoro-D-glucose (FGIc), D-glucosamine (GIcN), and tunicamycin (Tun) are given. The determination of the inhibition of the production of virus particles of enveloped viruses is a good method to assess the extent of inhibition of protein glycosylation. The specificity of the inhibition can be established by measuring the incorporation of [14C]asparagine into protein, which should not be decreased; in addition, the energy charge may be determined.
Publication Year: 1982
Publication Date: 1982-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 47
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