Title: A pause site for RNA polymerase II is associated with termination of transcription.
Abstract: Research Article1 July 1991free access A pause site for RNA polymerase II is associated with termination of transcription. P. Enriquez-Harris P. Enriquez-Harris Sir William Dunn School of Pathology, University of Oxford, UK. Search for more papers by this author N. Levitt N. Levitt Sir William Dunn School of Pathology, University of Oxford, UK. Search for more papers by this author D. Briggs D. Briggs Sir William Dunn School of Pathology, University of Oxford, UK. Search for more papers by this author N.J. Proudfoot N.J. Proudfoot Sir William Dunn School of Pathology, University of Oxford, UK. Search for more papers by this author P. Enriquez-Harris P. Enriquez-Harris Sir William Dunn School of Pathology, University of Oxford, UK. Search for more papers by this author N. Levitt N. Levitt Sir William Dunn School of Pathology, University of Oxford, UK. Search for more papers by this author D. Briggs D. Briggs Sir William Dunn School of Pathology, University of Oxford, UK. Search for more papers by this author N.J. Proudfoot N.J. Proudfoot Sir William Dunn School of Pathology, University of Oxford, UK. Search for more papers by this author Author Information P. Enriquez-Harris1, N. Levitt1, D. Briggs1 and N.J. Proudfoot1 1Sir William Dunn School of Pathology, University of Oxford, UK. The EMBO Journal (1991)10:1833-1842https://doi.org/10.1002/j.1460-2075.1991.tb07709.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Termination of transcription by RNA polymerase II has been postulated to involve a pausing process. We have identified such a pause signal, 350 bp into the 3′ flanking region of the human alpha 2 globin gene at a position where termination is thought to occur. We show that this pause signal enhances the utilization of an upstream poly(A) site which is otherwise out-competed by a stronger downstream poly(A) site. We also demonstrate that the pause site rescues a poly(A) site that is inactive due to its location within an intron. Using nuclear run-on analysis we show that elongating RNA polymerase II molecules accumulate over this pause signal. Furthermore we show that when the pause site is positioned immediately downstream of a strong poly(A) signal, significant levels of transcription termination take place. Previous ArticleNext Article Volume 10Issue 71 July 1991In this issue RelatedDetailsLoading ...