Title: Short N-terminal regions of the calcitonin-like receptor define ligand specificity
Abstract:The calcitonin (CT)-like (CL) receptor is a CT gene-related peptide (CGRP) receptor or an adrenomedullin (AM) receptor when coexpressed with receptor-activity-modifying proteins (RAMP) 1 or –2, respec...The calcitonin (CT)-like (CL) receptor is a CT gene-related peptide (CGRP) receptor or an adrenomedullin (AM) receptor when coexpressed with receptor-activity-modifying proteins (RAMP) 1 or –2, respectively. The CL receptor shows 57% overall sequence identity with the CT receptor, but the homology is much lower in the extreme Nterminus. An N-terminal deletion mutant of the human (h) CL receptor (∆18-hCL) and a chimeric receptor consisting of the N-terminal amino acids of the porcine (p) CT receptor fused to the ∆18-hCL receptor (pCThCL) were therefore analyzed. The ∆18-hCL receptor function was abolished when co-expressed with RAMP1 or -2. The pCT-hCL receptor was a fully functional CGRP receptor when co-expressed with RAMP1, but the RAMP2-dependent AM receptor function was impaired. Limited sequence similarities in the N-terminus of the pCT and the hCL receptors rescue CGRP but not AM receptor binding and signalling.Read More
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: dissertation
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