Title: [27] B cell growth and differentiation factors
Abstract: This chapter provides an overview of B cell growth and their differentiation factors. The growth and differentiation of B lymphocytes is mediated by soluble products released by T cells and macrophages. B cell trophic cytokines include interleukin-1 (IL-1) which is secreted by macrophages, B cell stimulatory factor I (BSF-1; previously known as B cell growth factor I or BCGF I), B cell growth factor II (BCGF II), T cell replacing factor (TRF), interleukin-2 (IL-2), and several B cell differentiation and maturation factors (BCDF or BMF) which are secreted by T cells. The chapter further discusses preparation of supernatants containing BSF-1. BSF-l-containing supernatants are derived from T cells stimulated either with mitogens, or phorbol myristate acetate (PMA). The initial step in purifying BSF-1 involves concentration of crude supernatants by ammonium sulfate precipitation. The bulk of the BSF-1 activity is found in the 50-100% saturated ammonium sulfate fractions. The next purification step varies among laboratories but involves either Phenyl Sepharose chromatography. In addition, BCGF II is also described with its purification.
Publication Year: 1985
Publication Date: 1985-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 12
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