Publication Year: 2000
DOI: https://doi.org/10.1083/jcb.148.4.635
Abstract: An understanding nucleocytoplasmic provide evidence for a Brownian affinity gating mechanism for nucleocytoplasmic exchange transport. requires a comprehensive inventory of the molecular components of of the NPC and a knowledge of how each component how contributes to the overall structure of this large molecular translocation the machine. Therefore, we have Show more
Authors:
Publication Year: 2007
DOI: https://doi.org/10.1038/nature06405
Abstract: Not available
Authors:
Publication Year: 2002
DOI: https://doi.org/10.1083/jcb.200206106
Abstract: As the complex nucleoporins were six previously undiscovered nucleoporins and a novel family (NPC) of WD repeat nucleoporins. One of these WD repeat nucleoporins has is ALADIN, the gene mutated in triple-A (or Allgrove) syndrome. a Our analysis defines the proteome of the mammalian NPC for vital the first time Show more
Authors:
Publication Year: 2017
DOI: https://doi.org/10.1016/j.cell.2017.10.008
Abstract: Not available
Authors:
Publication Year: 2002
DOI: https://doi.org/10.1091/mbc.01-06-0308
Abstract: Bidirectional transport occurs 39 nm could be translocated by the NPC. This implies through that macromolecules much larger than the assumed functional NPC diameter the of 26 nm can be transported into the karyoplasm. The nuclear physiological relevance of this finding was supported by the observation pore that intact nucleocapsids Show more
Authors:
Publication Year: 2011
DOI: https://doi.org/10.1146/annurev-biochem-060109-151030
Abstract: In eukaryotic in concepts of its underlying architecture, and discuss key outstanding questions the and challenges. The applied structure determination as well as the nucleus described design principles of the NPC may serve as paradigms and for other macromolecular assemblies. translation in the cytoplasm imposes the cells, requirement of transporting Show more
Authors:
Publication Year: 2010
DOI: https://doi.org/10.1101/cshperspect.a000562
Abstract: Susan R. and Developmental Biology, Vanderbilt University Medical Center, Nashville, Tennessee 37232 Wente1 Laboratory of Cellular and Structural Biology, The Rockefeller University, New and York, New York 10065 Correspondence: susan.wente{at}vanderbilt.edu and rout{at}mail.rockefeller.edu Michael P. Rout2 Department of Cell
Authors:
Publication Year: 2001
DOI: https://doi.org/10.1093/emboj/20.6.1320
Abstract: Not available
Authors:
Publication Year: 1997
DOI: https://doi.org/10.1016/s0092-8674(00)81862-0
Abstract: Not available
Authors:
Publication Year: 1996
DOI: https://doi.org/10.1083/jcb.135.6.1457
Abstract: Ran is the fibers of the NPC. The modified form of RanGAP1 also bidirectional appeared to associated with the mitotic spindle apparatus during mitosis. transport These findings have specific implications for Ran function and broad of implications for protein regulation by ubiquitin-like modifications. Moreover, the variety proteins and function of Show more
Authors:
Publication Year: 2014
DOI: https://doi.org/10.1073/pnas.1406459111
Abstract: Significance Proximity-dependent identifying have measured the labeling radius of BioID, thus enabling the protein rational application of this method and more meaningful data interpretation. associations that occur in living cells. Fusion of biotinylation a promiscuous biotin ligase to a bait protein for expression (BioID) in live cells enables covalent biotin Show more
Authors:
Publication Year: 2004
DOI: https://doi.org/10.1126/science.1104808
Abstract: Nuclear pore analyze their structure in a close-to-life state, we studied transport-active, complexes intact nuclei from Dictyostelium discoideum by means of cryoelectron tomography. (NPCs) Subvolumes of the tomograms containing individual NPCs were extracted in are silico and subjected to three-dimensional classification and averaging, whereby distinct gateways structural states were observed. Show more
Authors:
Publication Year: 1986
DOI: https://doi.org/10.1016/0092-8674(86)90784-1
Abstract: Not available
Authors:
Publication Year: 1987
DOI: https://doi.org/10.1083/jcb.104.5.1143
Abstract: Using monoclonal of When the nuclear envelope is disassembled and subsequently reformed during rat mitosis, the proteins are reversibly dispersed throughout the cytoplasm in liver the form of minute foci. By EM immunogold localization on nuclear isolated nuclear envelopes, the monoclonal antibodies label exclusively the nuclear envelopes pore complex, at Show more
Authors:
Publication Year: 1987
DOI: https://doi.org/10.1083/jcb.104.2.189
Abstract: Selective transport biochemical by subsequent addition of sugar. When rat liver nuclei that differences had been incubated with ferritin-labeled WGA were examined by electron are microscopy, multiple molecules of WGA were found bound to the maintained cytoplasmic face of each nuclear pore. Gel electrophoresis and nitrocellulose between transfer identified one Show more
Authors:
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