Title: Cloning of genes involved in pathogenicity of <i>Xanthomonas campestris</i> pv. <i>campestris</i> using the broad host range cosmid pLAFR1
Abstract: Article20 December 1984free access Cloning of genes involved in pathogenicity of Xanthomonas campestris pv. campestris using the broad host range cosmid pLAFR1 M.J. Daniels M.J. Daniels John Innes Institute, Colney Lane, Norwich NR4 7UH, UK Search for more papers by this author C.E. Barber C.E. Barber John Innes Institute, Colney Lane, Norwich NR4 7UH, UK Search for more papers by this author P.C. Turner P.C. Turner John Innes Institute, Colney Lane, Norwich NR4 7UH, UK Search for more papers by this author M.K. Sawczyc M.K. Sawczyc John Innes Institute, Colney Lane, Norwich NR4 7UH, UK Search for more papers by this author R.J.W. Byrde R.J.W. Byrde John Innes Institute, Colney Lane, Norwich NR4 7UH, UK Long Ashton Research Station, Long Ashton, Bristol BS18 9AF, UK Search for more papers by this author A.H. Fielding A.H. Fielding John Innes Institute, Colney Lane, Norwich NR4 7UH, UK Long Ashton Research Station, Long Ashton, Bristol BS18 9AF, UK Search for more papers by this author M.J. Daniels M.J. Daniels John Innes Institute, Colney Lane, Norwich NR4 7UH, UK Search for more papers by this author C.E. Barber C.E. Barber John Innes Institute, Colney Lane, Norwich NR4 7UH, UK Search for more papers by this author P.C. Turner P.C. Turner John Innes Institute, Colney Lane, Norwich NR4 7UH, UK Search for more papers by this author M.K. Sawczyc M.K. Sawczyc John Innes Institute, Colney Lane, Norwich NR4 7UH, UK Search for more papers by this author R.J.W. Byrde R.J.W. Byrde John Innes Institute, Colney Lane, Norwich NR4 7UH, UK Long Ashton Research Station, Long Ashton, Bristol BS18 9AF, UK Search for more papers by this author A.H. Fielding A.H. Fielding John Innes Institute, Colney Lane, Norwich NR4 7UH, UK Long Ashton Research Station, Long Ashton, Bristol BS18 9AF, UK Search for more papers by this author Author Information M.J. Daniels1, C.E. Barber1, P.C. Turner1, M.K. Sawczyc1, R.J.W. Byrde1,2 and A.H. Fielding1,2 1John Innes Institute, Colney Lane, Norwich NR4 7UH, UK 2Long Ashton Research Station, Long Ashton, Bristol BS18 9AF, UK The EMBO Journal (1984)3:3323-3328https://doi.org/10.1002/j.1460-2075.1984.tb02298.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info A genomic library was prepared in Escherichia coli from DNA of wild-type Xanthomonas campestris pv. campestris (aetiological agent of crucifer black rot), partially digested with endonuclease EcoRI, using the mobilisable broad host range cosmid vector pLAFR1. Recombinant plasmids contained inserts ranging in size from 19.1 to 32.3 kb (mean 26.6). Certain of the clones complemented E. coli auxotrophic markers. Using the narrow host range plasmid pRK2013 as a helper the pooled recombinant plasmids were transferred conjugally to X. c. campestris mutants, and clones were identified which restored yellow pigmentation to white mutants, prototrophy to amino acid auxotrophs and pathogenicity towards turnip plants to two non-pathogenic mutants. The lesion in one mutant (8288, complemented by the plasmid pIJ3000) is unknown. However mutant 8237 is defective in production of extracellular protease and polygalacturonate lyase and restoration of pathogenicity by complementation with the plasmid pIJ3020 concomitantly restored both enzyme levels to wild-type values. Previous ArticleNext Article Volume 3Issue 131 December 1984In this issue RelatedDetailsLoading ...