Title: Histomorphometry of umbilical cord and its vessels in natural and assisted reproduction
Abstract: Introduction and Aim: Assisted reproductive technique (ART) is an invaluable gift for infertile couple who failed in various infertility treatments. However, there are many aftereffects after assisted reproduction like preterm birth, small for gestational age, congenital anomalies etc. Umbilical cord is of fetal origin, which connects the fetus to placenta. Therefore, changes in the histomorphometry of umbilical cord and its vessels may affect the fetus and its growth. Our present study aimed to analyze the histo-morphometric parameters of umbilical cord and its vessels in natural conception and in assisted reproduction.
Materials and Methods: 30 placentas (with umbilical cord) of natural conception and 30 placentas (with umbilical cord) of assisted reproduction (ICSI) were collected immediately after delivery in and around Chidambaram. Umbilical cord bits were fixed in buffered formalin and underwent routine tissue processing procedure. 5 to 10 microns of tissue sections were taken and stained in H & E stain, Verhoeff stain and MTS stain. Histo- morphometric parameters of umbilical cord and its vessels were measured in Olympus microscope using ocular micrometer.
Results: There was a reduction in the areas of umbilical cord and its vessels in assisted reproduction when compared to natural conception.
Conclusion: These results would be useful for neonatologists who handle the newborns of ART mothers.
Keywords: Umbilical cord; umbilical artery; umbilical vein; histomorphometry; connective tissue.
Publication Year: 2019
Publication Date: 2019-01-01
Language: en
Type: article
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