Title: Mechanism of As_2O_3 on hdpr1 Gene Demethylation in Jurkat Cell Line
Abstract: This study was purposed to investigate the effect of As2O3 on the demethylation of anti-oncogene-hdpr1 gene of acute lymphoblastic leukemia cell line Jurkat in vitro and its mechanism.The inhibitory effect of As2O3 on the proliferation of Jurkat cells was assayed by CCK-8; the change of Jurkat cell cycle was detected by flow cytometry before and after using As2O3; the effect of As2O3 on the methylation model of hdpr1 gene was analyzed by methylation-specific PCR,and the effect of As2O3 on the expression of hdpr1 mRNA was analyzed by semiquantitative RT-PCR. The results showed that the proliferation rate of Jurkat cells was decreased significantly after being treated with As2O3,and in dose-and time-dependent manner; As2O3 blocked Jurkat cell cycle in G0/G1 phase in dose-dependent manner. As2O3 could reverse hypermethylation of hdpr1 gene and induce its mRNA reexpression,and down-regulate the dnmt1,dnmt3a,dnmt3b mRNA expression level also in dose-dependent manner. It is concluded that the As2O3 suppresses the proliferation of Jurkat cells and blocks cell cycle is G0/G1,its possible mechanism may be down-regulating mRNA expression level of dnmt1,dnmt3a and dnmt3b,induc demethylation of hdpr1 gene from abnormal hypermethylation status and activates its reexpression.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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