Title: Studies on genetic diversity and phylogeny of slow_growing rhizobia isolated from Vigna radiata at main ecotypes of China
Abstract: Studies on genetic diversity and phylogeny of slow_growing rhizobia isolated from Vigna radiata at main ecotypes of China were conducted by using 16S rRNA gene PCR_RFLP, 16S rRNA gene sequencing and 16S_23S rRNA IGS PCR_RFLP assays. Results of 16S rRNA gene PCR RFLP analysis reveal that all the strains tested are clustered into three groups at the similarity of 76%. GroupⅠcontains 13 slow_growing rhizobia tested including LYG1; Group Ⅱ consists of 21 strains tested and the type strains of B. japonicum and B.liaoningense, and 10 tested strains isolated from Guangdong,Guangxi and the type strain of B. elkanii.compose Group Ⅲ. The results of 16S_23S rRNA IGS PCR_RFLP show that strains tested could be divided into A and B groups, which could be correspondently subdivided into AⅠ, AⅡ, AⅢ, BI and BⅡ subgroups at the similarity of 85%. Compared with 16S rRNA PCR_RFLP, IGS RFLP assay show higher resolution, strains and reference strains tested can be divided into 21 IGS RFLP patterns. The strains isolated from Xinjiang, Guangdong and Guangxi regions show obvious geographical effect on genetic diversity.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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