Title: Change in expression of macrophage migration inhibitory factor mRNA in a rat model of ventilator-induced lung injury
Abstract: Objective To investigate the changes in the expression of macrophage migration inhibitory factor (MIF) mRNA in a rat model of ventilator-induced lung injury. Methods Thirty adult male Sprague-Dawley rats, weighing 235-260 g, were randomly divided into 3 groups ( n = 10 each) using a random number table: con- trol group (group C), small tidal volume (VT ) mechanical ventilation group (group S) and large tidal volume me- chanical ventilation group (group L) . The animals were anesthetized with intraperitoneal ketamine 100 mg/kg, mi- dazolam 0.2 mg/kg and atropine 1.0 mg/kg. The rats were tracheostomized and spontaneous breathing was main- tained in group C, while the rats were tracheostomized and mechanically ventilated for 4 h in groups S and L. The tidal volume was 7 ml/kg (group S) or 40 ml/kg (group L),I: E was 1 : 1, RR was 80 bpm and FiO2 was 100% . At 4 h of spontaneous breathing or mechanical ventilation, broncho-alveolar lung lavage fluid (BALF) was collect- ed for determination of the total protein concentration; white blood cell (WBC) counts and concentrations of MIF, IL-6 and IL-1β (by ELISA). Then the rats were sacrificed and the lungs removed for microscopic examination and for determination of wet to dry lung weight ratio (W/D ratio) and expression of MIF mRNA (by RT-PCR). Results Compared with C and S groups, WBC counts, concentrations of total protein, MIF, IL-6 and IL-1β in BALF, and W/D ratio and expression of MIF mRNA in lung tissues were significantly increased in group L (P 〈 0.05). There was no significant difference in the indexes mentioned above between group C and group S ( P 〉 0.05 ). The pathological changes occurred in group L. Conclusion The up-regulation of MIF mRNA expression in lung tissues may be involved in the development of ventilator-induced lung injury in rats.
Key words:
Respiration, artificial; Respiratory distress syndrome, adult; Macrophage migration-in-
Publication Year: 2013
Publication Date: 2013-11-20
Language: en
Type: article
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