Title: Preparative asymmetric reduction of ketones in a biphasic medium with an (S)-alcohol dehydrogenase under in situ-cofactor-recycling with a formate dehydrogenase
Abstract: The substrate range of a novel recombinant (S)-alcohol dehydrogenase from Rhodococcus erythropolis is described. In addition, an enzyme-compatible biphasic reaction medium for the asymmetric biocatalytic reduction of ketones with in situ-cofactor regeneration has been developed. Thus, reductions of poorly water soluble ketones in the presence of the alcohol dehydrogenase from R. erythropolis and a formate dehydrogenase from Candida boidinii can be carried out at higher substrate concentrations of 10–200 mM. The resulting (S)-alcohols were formed with moderate to good conversion rates, and with up to >99% ee.
Publication Year: 2003
Publication Date: 2003-12-17
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 104
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