Title: Phylogenic Study of Rhizobia Strains Isolated from Rice Bean (Vigna umbellata L.) in Hubei by 16S-23S rDNA RFLP and 16S rRNA Gene Sequencing
Abstract: The genetic diversity and phylogeny of the rhizobia isolated from nodules of rice bean (Vigna umbellata L.) grown in Hubei were studied using 16S-23S rDNA intergenic spacer (IGS) PCR-RFLP and partial 16S rRNA gene sequencing. The result of 16S-23S rDNA IGS PCR-RFLP revealed that all the strains isolated from rice bean nodules could be divided into two groups at the similarity level of 52%. Group Ⅰ was composed of slow-growing strains and could be further divided into two branches, ⅠA and ⅠB at the similarity level of 79%. Group Ⅱ was composed of fast-growing strains, which also could be further divided into two branches, ⅡA and ⅡB at the similarity level of 62%. Branch ⅡA consisted of ⅡA1, ⅡA2 and ⅡA3 clusters at the similarity level of 72%. The strains of branch IIB were highly related to Sinorhizobium fredii USDA205T. Sequence analysis of partial 16S rRNA gene showed that 4 strains fell into different phylogenetic branches. CYR4243 shared 99.87% sequence homology with S. fredii type strain USDA205T. HCY1101 was highly related to Rhizobium leguminosarum bv. trifolii and R. leguminosarum bv. viceae. HCY5202 shared 99.86% sequence homology with R. galegae, and there was a high sequence homology between strain CYY3302 and Bradyrhizobium elkanii. Fig 4, Tab 1, Ref 13
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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