Title: Expression of apoptosis-associated proteins and drug resistance in patients with ovarian cancer
Abstract: [Objective] This study was designed to explore the role of apoptosis- associated genes and proteins on the chemo-resistance in COC1/DDP. [Methods] The apoptotic ratios of COC1 and COC1/DDP were measured with flow cytometry after treated with different concentration of cisplatin for 48 hours. The expressions of apoptosis- associated genes and proteins Bcl-2, Caspase-3, Smac, Survivin were studied by reverse trans-cription polymerase chain reaction (RT-PCR) and Western blot in COC1/ DDP and COC1. [Results] Using DDP to stimulate COC1/DDP and COC1 for 48 hours, the apoptic ratios were gradually increased with the increase of concentration of DDP. The mRNA and protein expressions of Bcl-2 and Survivin in COC1/DDP cells were significantly higher than those in COC1 cells (P 0.05), contrastly Smac and Caspase-3 were significantly lower than those in COC1 cells. [Conclusion] The cisplatin-resistance in human ovarian carcinoma cell lines may be associated with the overexpression of anti-apoptic gene and protein of Bcl-2, Survivin and the downregulation of Caspase-3 and Smac.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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