Title: Effects of Sodium Arsenite on Transcription and Expression of DNA Methyltransferase 1(DNMT1) and Histone Deacetylase 1(HDAC1) Gene in HaCaT Cells
Abstract: Objective To observe the influences of different doses of sodium arsenite on mRNA transcription and protein expression of DNA methyltransferase 1(DNMT1) and histone deacetylase 1(HDAC1) gene in HaCaT cells.Methods HaCaT cells were treated with NaAsO2 at the doses of 0,3.13,6.25,12.5,25 μmol/L,every other day,24 h one time,for three times.The mRNA transcription of DNMT1 and HDAC1 was detected by real-time quantitative PCR,the protein expression of DNMT1 and HDAC1 was detected by Western blot.Human epidermal squamous carcinoma cell line A431 cells were set as the positive control group.Results Among the groups of HaCaT cells treated by 0,3.13,6.25,12.5 and 25μmol/L NaAsO2,the level of DNMT1 mRNA transcription were 0.650 90±0.174 05,0.930 53±0.145 56,0.752 93±0.244 62,0.558 11±0.051 02 and 0.370 17±0.08 84,the differences were significant(F=6.539,P0.05);the level of HDAC1 mRNA transcription were 2.02180±0.179 57,1.496 98±0.107 55,1.303 24±0.152 08,1.037 75±0.204 88 and 0.816 74±0.153 12,the differences were significant(F=17.914,P0.05).The level of DNMT1 protein expression were 0.000 87±0.000 10,0.026 04±0.002 63,0.283 19±0.072 62,0.842 61±0.082 39 and 1.579 87±0.200 83,the differences were significant(F=27.799,P0.05);the level of HDAC1 protein expression were 0.034 65±0.012 03,0.273 65±0.012 02,0.634 90±0.239 76,0.775 03±0.126 51 and 1.126 16±0.167 52,the differences were significant(F=9.820,P0.05).Conclusion DNMT1 and HDAC1 protein high expression is the important molecular event of arsenic-induced skin lesion,which may provide the possibility for using its inhibitors to explore the arsenic poisoning prevention and treatment.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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Cited By Count: 1
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