Title: Relationship of Starch Synthesis with It’s Related Enzymes’Activities During Rhizome Development of Lotus(Nelumbo nucifera Gaertn)
Abstract: 【Objective】The objective of this paper is to study the relationship of starch contents with its related key synthease’s activities in lotus rhizome.【 Method 】 Using four lotus cultivars,the activity changes of adenosine diphosphoglucose pyrophosphorylase(ADPGPase),starch synthase(SSase) and starch branching enzyme(Q-enzyme) in rhizome during the development and their relationships with starch synthesis were studied.【Result】The peaks of fructose,glucose and total soluble sugar,sucrose content reached at early,middle,end stage of rhizome development,respectively.Total starch,amylose and amylopectin content in rhizome increased quickly after middle stage,total starch content reached 11.1% to 12.7% in fresh rhizome at end stage,and 70% of total starch was amylopectin.ADPGPase and SSase activities at the same development stage in different cultivar’s rhizome were obviously different,but Q-enzyme activity had little difference.During rhizome development SSase activity peak appeared earlier than ADPGPase and Q-enzyme at early stage,while ADPGPase and Q-enzyme activity peaks appeared at middle stage.ADPGPase activity at middle stage and SSase activity at early stage were very significantly and significantly correlated with total starch content at end stage in rhizome,co-efficients were 0.9830(P 0.01;n=12) and 0.8458(P0.05;n=12),respectively.Q-enzyme activity at middle stage was significantly correlated with amylopectin content at end stage,with co-efficient 0.7690(P 0.05;n=12).【Conclusion】Total starch content in matured lotus rhizome was determined by ADPGPase and SSase activities at middle and early stage;amylopectin content was determined by Q-enzyme activity at middle stage during lotus rhizome development.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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Cited By Count: 13
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