Title: Estrogen receptor β1 induces apoptosis of MDA-MB-231 breast cancer cells by regulating the expression of Bax gene
Abstract: Objective To observe the changes in Bax and Bcl-2 expressions and to investigate the biology role of estrogen receptor(ER)β1 in MDA-MB-231 breast cancer cells by using RNA interference to interrupt ERβ1 expression.Methods pSilencer-ERβ1 was transfected into MDA-MB-231 breast cancer cells by using cationic liposome Lipofectamine 2000TM as tranfecting agent.After transfection,the expression levels of mRNA and protein of ERβ1,Bcl-2 and Bax were evaluated by real-time polymerase chain reaction(RT-PCR)and Western blot,respectively.Cell apoptosis was detected using flow cytometry.Cell growth curve was performed to show the changes in proliferation ability.Comparison between quantitative data was performed sing t test and one factor analysis of variance.Results After ERβ1 siliencing,both mRNA and protein level of ERβ1 and Bax gene decreased significantly compared with the control groups(P0.05),and cell growth curve showed a marked enhancement in cell proliferation(P0.05).In the pSilencer-ERβ1 transfection group,there was an obvious decrease in mRNA and protein level of Bax gene(P0.01);no significant change in the expression of Bcl-2;and the ratio of Bcl-2/Bax increased.The results from flow cytometry showed that cell apoptosis decreased markedly in the pSilencer-ERβ1 transfection group compared with the controls(t=6.22,P=0.00).Conclusion ERβ1 could induce apoptosis of MDA-MB-231 breast cancer cells by regulating Bax gene expression,which may play an important role in ERβ1 inhibiting the growth of MDA-MB-231 breast cancer cells.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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