Title: Synthesis and identification of Ofloxacin artificial antigen
Abstract: Ofloxacin(OFL) was coupled with carrier protein bovine serum albumin(BSA) and ovalbumin(OVA) to prepare immunizing antigen and OFL-BSA and coating OFL-OVA by modified method of carbodiimide. UV scanning spectrum and SDS-PAGE indicated that the artificial antigens both OFL-BSA and OFL-OVA were successfully coupled. BALB/C mice were immunized with OFL-BSA, the titre of polyclonal antibody(pAb) was detected by indirect ELISA, the sensitivity of pAb was identified by blocking ELISA and the specificity of pAb was identified by the method of cross-reaction test. The results showed that sensitive and specific anti-OFL pAb have been produced in sera of immunized BALB/C mice, which made it significant to prepare monoclonal antibody of Ofloxacin.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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