Title: Role of neutrophil apoptosis in the lung of rats with acute lung injury
Abstract: Objective To investigate the potential role of neutrophil(PMN) apoptosis in bronchoalveolar lavage fluid (BALF) in the development of acute lung injury (ALI). Method Thirty SD rats were randomly divided into two groups ALI group and sham operation group.The parameters of ALI including pulmonary pathology, lung injury scores (LIS), lung water content (wet/dry, W/D), lung permeability index (LPI), tumor necrosis factor-α(TNF-α) were observed.Apoptosis was detected by flow cytometry. Result It was observed that alveolar spaces were narrow and neutrophils exuded at 2 hours,the pulmonary damage was worsened with prolongation of time. LIS increased at 2 hours and reached the peak at 16 hours, the differences were significant between ALI group and sham operation group (all P0.05), there was no significant defference in LIS among all the groups of ALI. W/D increased and the differences were significant between ALI group and sham operation group (all P0.05). LPI increased, the differences were significant between 6,8 and 16 hours group in ALI and sham operation group (all P0.01), but there was no significant difference between 2,4 hours group in ALI and sham operation group (all P0.05). TNF-α increased in ALI group and reached the peak at 2 hours, the differences were significant between 2, 4 and 6 hours group in ALI and sham operation group (all P0.05), but there was no significant differences between 8, 16 hours group and sham operation group (all P0.05). PMN apoptotic cell decreased and reached the low at 2 hours,the differences were significant between ALI group and sham operation group (all P0.01).Conclusion PMN apoptosis may play an important role in the pathogenesis of ALI. With prolongation of ALI, PMN apoptosis declined,and kept a low level.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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Cited By Count: 1
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