Title: Mechanism of pulsed magnetic field on reversal of multi-drug resistance of the leukemic cell HL60/ADR
Abstract: Objective:To explore effect of Retinoic Acid(RA) on proliferation of human osteosarcoma MG-63 cells and its mechanism of antitumor.Methods:The MG-63 cells treated by 1μmol/L RA were subjected to growth curve counts,flow cytometry,immuncocytochemical assay and light microscopy.We investigated the influence of RA on proliferation,cellcycle,expression of associated oncogene and tumor suppressor gene of MG-63cells.Then we analyzed the mechanism of RA in inducing differentiation primarily.Results:Seven days after treated by 1 μmol/LRA,the inhibition rate of proliferation of MG-63 cells amounted to 42.2 % and RA reduced the rate of progression from G1 to S phase.The cells tended to be flat and spread.Meanwhile,the expression level of oncogene was downregulated and the expression level of tumor suppressor gene was upregulated.Conclusion:1μmol/L RA could inhibit proliferation of MG-63cells,reverse the malignant phenotype characters,downregulate expression of oncogene c-Myc,c-fos and upregulate expression of Tumor Suppressor Gene Rb,p27,as a result,induce MG-63 cells into differentiation effectively.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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Cited By Count: 1
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