Title: Effect of recombinant adenovirus-p53 combined with oxaliplatin on the growth inhibition of human gastric cancer cell line BGC-823
Abstract: Objective To observe the effect of recombinant adenovirus-p53(rAd-p53) combined with oxaliplatin on the growth inhibition of human gastric cancer cell line BGC-823.Methods The human gastric cancer cell line BGC-823 was treated with rAd-p53(group A),oxaliplatin(group B) or combined rAd-p53 and oxaliplatin(group C) for different times.MTT assay was used to examine the suppression rate of cell growth.The p53 protein expression was detected by immunohistochemistry assay and expression of Caspase-3 in the cells was induced by flow cytometry.The results were compared to those in group D as the control.Results The cell growth inhibition rate was gradually increased as the drug concentration and time increased in groups of A and B.The cell growth inhibition rate was increased when the cells were treated with much lower concentration for 24 hours in group C(P0.05).Compared with group D,the content of Caspase-3 in gastric cancer cell was increased more(P0.05),but p53 protein was not(P0.05).Conclusion The chemotherapeutic sencitivity of oxaliplatin can be enhanced by rAd-p53,which may be related to induced apoptosis through mitochondrial pathway to activate downstream of Caspase-3.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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