Title: Cloning and analysis of a MADS-like gene in Carya cathayensis
Abstract: MADS-box family genes play key roles in all phases of floral development. Based on a partial fragment acquired from 454 sequencing of Carya cathayensis (hickory) floral buds, a full-length of a MADS-like gene was obtained by rapid amplification of cDNA ends (RACE). Afterwards, a quantitative real-time (qRT) polymerase chain reaction (PCR) analysis, an amino acid sequence alignment, and a yeast one-hybrid assay were performed. Results of the sequence analysis revealed the open reading frame (ORF) of the CcMADS-like gene was 609 bp in length and encoded a protein of 202 aa. The protein presented typical characteristic of MADS-box family genes and contained MADS domain, I, K, and C, with the MADS domain was considerably conserved. The amino acid sequence alignment showed that the putative protein CcMADS-like gene had an identity to homologies of Arabidopsis thaliana (48%), Antirrhinum majus (65%), Populus deltoides (69%), and Populus trichocarpa (71%). The qRT-PCR analysis revealed that the expression level of CcMADS-like gene in flower buds was much higher than in other tissues. The yeast one-hybrid showed that the CcMADS-like gene had an activation activity as a transcription factor. Overall, the qRT-PCR analysis suggested that CcMADS-like gene possibly participated in floral development of hickory. [Ch, 5 fig. 1 tab. 25 ref.]
Publication Year: 2015
Publication Date: 2015-02-20
Language: en
Type: article
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