Title: Investigation on the Extraction Conditions of Nucleotide from Beer Yeast by High-concentration Salt Method
Abstract: The extraction conditions of nucleotide from beer yeast by high-concentration salt method were studied and the best extraction conditions were summed up as follows: temperature at 95 ℃,10 % salt addition level,120 min extraction time,adjustment of isoelectric point,pH value as 4.2,after protein precipitation,8 h stable standing at 4 ℃,and 0.5308 g RNA extracted from 45 g wet yeast(equal to 10 g dry yeast) with A260/A280 as 1.988 and purity over 90 %.(Tran.by YUE Yang)
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot