Title: Mechanism of Direct Redifferentiation of Flower Bud from Explant of Dianthus chinensis L. by Cytokinin Inducement
Abstract: Using the MS+2, 4-D 0. 1 mg/L as the basic culture medium to which different concenttation of zeatin, benzylaminopurine and kinetin were added. Direct differentiation of blade, stem and calyx explant to flower bud was studied. Blade explant could redifferentiate to red flower hod in medium containing benzylaminopurine 2 mg/L (Fig. 1). But in the medium containing zeatin 2 mg/L the blade explant redifferentiated to form flower bud with no leaf or one to two leaves.These flower budS could develop into hermaphroditic flower, pistillate flower or flower with single pistil.Hermaphroditic flower could form fruit which set seeds (Figs. 2~5 ). In medium containing Kinetin no flower bud was formed. The stem explants could not be induced to form flower buds in cyto kinin. The calyx explants redifferentiated to form flower buds with no leaf in medium containing kinetin 3 mg/L (Fig. 6), In medium containing zeatin or benzylaminopurine no flower bud was induced. The results show that the kind and dosage of hormone or growth regulating substance are dicisive factors in inducing flower differentiation. But the response to hormone or growth regulsting substance is different for different Parts of explants. On the other hand,if the blooming condition is given,the callus can by pass through the vegetative growing stage and form directly the reproductive sarge and differentiate to form flower bud with no leaf.
Publication Year: 1995
Publication Date: 1995-01-01
Language: en
Type: article
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Cited By Count: 2
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