Title: Influence of dredging large intestine on content of cytokines in rats with chronic obstructive pulmonary disease
Abstract: Objective To observe the influences of dredging large intestine on content changes of interleukin-8(IL-8),interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α) and interleukin-10(IL-10) in peripheral blood and bronchoalveolar lavage fluid(BALF) in rats with chronic obstructive pulmonary disease(COPD),and investigate the mechanism of treating COPD from intestine from the angle of inflammation controlling.Methods The rat model of COPD was established by using intratracheal injection of LPS combining cigarette smoking.All rats were randomly divided into normal group,model group,treating intestine group(intestine group),treating lung group(lung group) and treating lung and intestine group(lung-intestine group).Normal group and model group were intragastrically given purified water and other groups were given corresponding Chinese medicinals for 14 days.The content of IL-8,IL-1β,TNF-αand IL-10 in peripheral blood and BALF were detected by applying enzyme-linked immunosorbent assay(ELISA).Results ①Compared with normal group,the content of IL-8,IL-1β,TNF-α and IL-10 in peripheral blood and BALF all increased in intestine group(P0.01 or P0.05).②Compared with model group,the content of IL-8,IL-1β,TNF-α and IL-10 in peripheral blood and BALF decreased in intestine group(P0.01 or P0.05).③Compared with lung group,the content of IL-1β in peripheral blood and IL-8 in BALF decreased in lung-intestine group(P0.01 or P0.05).Conclusion Dredging large intestine or dredging large intestine based on treating lung can reduce the content of cytokines in peripheral blood and BALF to relieve general or local inflammatory reaction in rat with COPD,which may be one of effects of treating COPD from intestine.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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