Title: Agrobacterium Tumefaciens-mediated Transformation of the Penicillium Simplicissimum H5
Abstract: Agrobacterium tumefaciens-mediated transformation(ATMT)was applied in Penicillium simplicissimum H5 by Agrobacterium tumefaciens LBA4404.The results of hygromycin-resistance selection,PCR and Southern blot analysis showed that T-DNA was randomly inserted into the transformants with single copy and the transformants indicated genetic stability.The transformation efficiency was about 50 transformants per 105 spores by optimizing the co-culture time,the concentration of Agrobacterium tumefacien and acetoyringone(AS).The application of ATMT technique in Penicillium simplicissimum H5 will provide a powerful tool for the genetic engineering of this fungus.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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