Title: Trichostatin A combined with docetaxel promote apoptosis of lung adenocarcinoma cells and the possible molecular mechanisms
Abstract: Objective:To explore the effects of trichostatin A(TSA) and docetaxel(Doc) on apoptosis of lung adenocarcinoma A549 cells and the possible molecular mechanisms.Methods: A549 cells were treated with TSA alone or in combination with Doc,MTT assay was used to measure the proliferation of A549 cells;cell morphological changes were observed under light microscope;apoptosis was assessed using Hoechst 33258 staining and flow cytometry;cell cycle was analyzed by flow cytometry;the expression of acetyl-α-tubulin and survivin protein and activation of caspase-3 were detected by Western blotting.Results: 10 μg/ml Doc and 250 nmol/L TSA alone or in combination significantly inhibited the growth of A549 cells in a time-dependent manner,and the combined treatment induced even higher inhibitory rate([65.6±3.1]% vs [30.6±2.1]%,[23.3±1.9]%,P0.05).In addition,TSA or Doc alone or in combination increased the apoptosis rate of A549 cells,and the combined treatment also induced higher apoptosis rate([58±3.6]% vs [17±2.2]%,[14±1.6]%,P0.05).The cell cycle was more markedly arrested in G2/M phase in combination treatment group([32.4±3.1]% vs [23.5±2.3]%,[10.5±1.5]%,P0.05),TSA combined with Doc increased the expression of acetyl-α-tubulin,reduced the expression of survivin and promoted the activation of caspase-3(P0.05).Conclusion: TSA combined with Doc can inhibit proliferation and induce apoptosis of A549 cells,which is related to the up-regulation of acetyl-α-tubulin,down-regulation of survivin and increased activation of caspase-3.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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