Title: Protective Effect of Intestinal Trefoil Factor on Colonic Damage of Mice with Inflammatory Bowel Disease
Abstract: Objective To explore the influence of intestinal trefoil factor(ITF) on colonic damage in mouse model with inflammatory bowel disease(IBD),and discuss its innate immune regulation on IBD. Methods Forty-eight mice were randomly assigned into 3 groups,group trinitrobenzenesulfonic acid(TNBS) and group recombinant ITF(rITF) as IBD groups,and the ethanol control group.After IBD models were established with TNBS,mice in rITF group were treated with rITF and mice in group TNBS were treated with 9 g·L-1 saline for 5 days,respectively.On the 14th day,signs of colitis,colonic damage score,the levels of toll-like receptor 4(TLR4),nuclear factor κBp65(NF-κBp65) mRNA and protein expression,and TNF-α level in colon were determined,respectively. Results Signs of colitis including weight loss,bloody stools,diarrhea,number of death,both microscopic and macroscopic injuries of the colon in rITF group were improved compared with those in group TNBS.Studies of immunohistochemistry and real time PCR demonstrated that the expressions of TNF-α,TLR4,NF-κBp65 production(P_a0.05) and TLR4,NF-κBp65 mRNA in rITF group significantly decreased compared with those in group TNBS(P_a0.01).There was no death mouse and little colonic damage in control group.The level of expression of tissue TNF-α,TLR4,NF-κBp65 production and TLR4,NF-κBp65 mRNA in TNBS group were increased markedly than those in control group(P_a0.01). Conclusion ITF may ameliorate IBD by protecting the colonic damage,and one of the mechanisms may related to innate immune regulation
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot