Abstract: HomeHypertensionVol. 47, No. 3Cardiac Glycosides and Cardiomyopathy Free AccessEditorialPDF/EPUBAboutView PDFView EPUBSections ToolsAdd to favoritesDownload citationsTrack citationsPermissions ShareShare onFacebookTwitterLinked InMendeleyReddit Jump toFree AccessEditorialPDF/EPUBCardiac Glycosides and Cardiomyopathy Paolo Manunta and Mara Ferrandi Paolo ManuntaPaolo Manunta From the Division of Nephrogy (P.M.), Dialysis and Hypertension, University "Vita-Salute" San Raffaele, Milan, and Prassis Sigma-Tau Research Institute (M.F.), Settimo Milanese, Italy. and Mara FerrandiMara Ferrandi From the Division of Nephrogy (P.M.), Dialysis and Hypertension, University "Vita-Salute" San Raffaele, Milan, and Prassis Sigma-Tau Research Institute (M.F.), Settimo Milanese, Italy. Originally published6 Feb 2006https://doi.org/10.1161/01.HYP.0000202641.29167.c0Hypertension. 2006;47:343–344Other version(s) of this articleYou are viewing the most recent version of this article. Previous versions: February 6, 2006: Previous Version 1 Digitalis-like steroids and related agents have been a mainstay in the treatment of congestive heart failure ever since the publication, in 1785, of Withering's seminal monograph on foxglove. Heart failure refers to the clinical syndrome that results when the heart is unable to pump sufficient blood to keep up with the metabolic demands of the body. Congestive heart failure is characterized by excessive neuronal and hormonal-mediated fluid retention, expanded intravascular volume, high pulmonary and systemic venous pressures with consequent dyspnea (shortness of breath) on exertion, reduced exercise tolerance, and fatigue. Most heart failure patients also have impaired ventricular systolic function and depressed cardiac output; these are the patients most often treated with digitalis glycosides. These drugs are positive inotropic agents and enhance cardiac contraction. The "cardiotonic steroids" cause cardiac muscle to lose K+ and gain Na+ because they inhibit the Na+ pump (Na+,K+–ATPase), a plasma membrane protein, present in all cells. The Na+ pump uses the energy from ATP to extrude Na+ and to maintain the large Na+ electrochemical gradient across the plasma membrane.How does inhibition of the Na+ pump augment cardiac contraction? The discovery of the Na+-Ca2+ exchanger and sarcoplasmic reticulum Ca2+ ATPase (Serca) has provided the missing link between Na+ pump inhibition and delivery of Ca2+ to enhance contractility.1 An increase of Na+-Ca2+ exchanger at the plasma membrane and/or a reduced Serca activity may contribute to increased intracellular Ca2+ concentrations leading to increased contractility. In 1997, the Digitalis Investigative Group reported on a controlled, randomized trial of digoxin in 6800 patients with congestive heart failure being treated with diuretics and angiotensin-converting enzyme inhibitors.2 The study led to the conclusion that digoxin therapy reduces hospitalization of patients affected by heart failure and confirmed the overall efficacy of digoxin therapy in patients with congestive heart failure and normal sinus rhythm. Yet, for unknown reasons, digoxin benefits only some patients. Clearly, some means of identifying the potential impact of digoxin before its administration would be extremely useful. The presence of endogenous cardiac glycosides, including marinobufagenin (MBG)3 and endogenous ouabain (EO)4 in the human circulation, should be taken into account. EO is not plant ouabain, but a related that remains structurally unclear compound.The article published in this issue of Hypertension by Kennedy et al5 is in keeping with the hypothesis of a new function of the endogenous cardiac glycosides. The authors investigated the role of marionobufagenin in uremic cardiomyopathic, suggesting that the increased levels of MGB are implicated in the development of a uremic cardiomyopathy associated with diastolic dysfunction, cardiac hypertrophy, and systemic oxidant stress. In particular the administration of 10 μg/kg per day of MBG for 4 weeks caused increased blood pressure, cardiac fibrosis, and systemic oxidant stress associated with decreased cardiac Serca2a expression. All these effects were attenuated following immunization against MBG.Evidence has been provided by this group, and by others, that cardiotonic steroids activate intracellular signaling pathways through Na/K-ATPase–Src–Ras-ROS and extracellular signal regulated kinase within restricted membrane subdomains, referred to as caveolae. This signal has genomic effects leading to changes in gene expression involved in the hypertrophic growth response. These data have shifted the focus of cardiotonic steroids from their pharmacological effects on the enzymatic function of the Na/K-ATPase to intracellular signaling functions triggering growth and cell proliferation.Similarly, longer term infusion of plant-derived ouabain (15 μg/kg per day for 18 weeks), at doses that just double the plasma levels (from 0.3 to 0.7 nmol/L), produced hypertension and cardiac hypertrophy in rats.6 Moreover, a growing number of studies suggest that elevated levels of endogenous cardiac glycosides, such as EO,4 may have a primary role in cardiac dysfunction and failure: In young offspring of hypertensive individuals, plasma EO levels positively correlate with certain indexes of diastolic cardiac dysfunction that precede the development of hypertension and left ventricular hypertrophy.7Approximately 30% of whites with uncomplicated new onset of essential hypertension show increased concentrations of EO associated with reduced heart rate and greater left ventricular mass and stroke volume.8Among patients with more advanced hypertension, the circulating levels of EO are directly related to both blood pressure and total peripheral resistance and inversely related to cardiac index.9Among patients with idiopathic dilated cardiomyopathy, high circulating levels of EO identify those individuals predisposed to progress more rapidly to heart failure.10 Moreover, patients on digoxin therapy display higher levels of EO compared with patients without therapy and this difference persist when all the confounders are take into account. This suggests that EO may contribute to digoxin toxicity.In agreement to these points, the study of Kennedy et al is extremely interesting in view of the fact that volume expansion appears to play a key role in cardiac hypertrophy associated with renal failure and MBG concentrations appear to increase following volume expansion. Taken together, these data strongly support an important role for endogenous glycoside, EO and MBG, in the pathogenesis of cardiomyopathy.The opinions expressed in this editorial are not necessarily those of the editors or of the American Heart Association.FootnotesCorrespondence to Paolo Manunta, MD, Division of Nephrogy, Dialysis and Hypertension, University "Vita-Salute" San Raffaele, Via Olgettina, 60, 20132 Milan, Italy. E-mail [email protected] References 1 Reuter H, Henderson SA, Han T, Ross RS, Goldhaber JI, Philipson KD. The Na+-Ca2+ exchanger is essential for the action of cardiac glycosides. Circ Res. 2002; 90: 305–308.CrossrefMedlineGoogle Scholar2 The Digitalis Investigative Group. The effect of digoxin on mortality and morbidity in patients with heart failure. N Eng J Med. 1997; 336: 525–533.CrossrefMedlineGoogle Scholar3 Fedorova OV, Talan MI, Agalakova NI, Lakatta EG, Bagrov AY. Endogenous ligand of alpha(1) sodium pump, marinobufagenin, is a novel mediator of sodium chloride-dependent hypertension. Circulation. 2002; 105: 1122–1127.CrossrefMedlineGoogle Scholar4 Hamlyn JM, Blaustein MP, Bova S, DuCharme DE, Harris DW, Mandel F. Identification and characterization of a ouabain-like compound from human plasma. Proc Natl Acad Sci U S A. 1991; 88: 6259–6263.CrossrefMedlineGoogle Scholar5 Kennedy DJ, Vetteth S, Periyasamy SM, Kanj M, Fedorova L, Khouri S, Kahaleh MB, Xie Z, Malhotra D, Kolodkin NI, Lakatta EG, Fedorova OV, Bagrov AY, Shapiro JI. Central role for the cardiotonic steroid marinobufagenin in the pathogenesis of experimental uremic cardiomyopathy. Hypertension. 2006; 47: 488–495.LinkGoogle Scholar6 Ferrandi M, Molinari I, Barassi P, Minotti E, Bianchi G, Ferrari P. Organ hypertrophic signaling within caveolae membrane subdomains triggered by ouabain and antagonized by PST 2238. J Biol Chem. 2004; 279: 33306–33314.CrossrefMedlineGoogle Scholar7 Manunta P, Iacoviello M, Forleo C, Messaggio E, Hamlyn JM, Lucarelli K, Guida P, Romito R, De Tommasi E, Bianchi G, Rizzon P, Pitzalis MV. High circulating levels of endogenous ouabain in the offspring of hypertensive and normotensive individuals. J Hypertens. 2005; 23: 1677–1681.CrossrefMedlineGoogle Scholar8 Manunta P, Stella P, Rivera R, Ciurlino D, Cusi D, Ferrandi M, Hamlyn JM, Bianchi G. Left ventricular mass, stroke volume, and ouabain-like factor in essential hypertension. Hypertension. 1999; 34: 450–456.CrossrefMedlineGoogle Scholar9 Pierdomenico SD, Bucci A, Manunta P, Rivera R, Ferrandi M, Hamlyn JM, Lapenna D, Cuccurullo F, Mezzetti A. Endogenous ouabain and hemodynamic and left ventricular geometric patterns in essential hypertension. Am J Hypertens. 2001; 14: 44–50.CrossrefMedlineGoogle Scholar10 Pitzalis MV, Hamlyn JM, Messaggio E, Iacoviello M, Forleo C, Romito R, de Tommasi E, Rizzon P, Bianchi G, Manunta P. Independent and incremental prognostic value of endogenous ouabain in idiopathic dilated cardiomyopathy. Eur J Heart Fail. 2005; [Epub ahead of print].Google Scholar eLetters(0)eLetters should relate to an article recently published in the journal and are not a forum for providing unpublished data. Comments are reviewed for appropriate use of tone and language. Comments are not peer-reviewed. Acceptable comments are posted to the journal website only. Comments are not published in an issue and are not indexed in PubMed. Comments should be no longer than 500 words and will only be posted online. References are limited to 10. Authors of the article cited in the comment will be invited to reply, as appropriate.Comments and feedback on AHA/ASA Scientific Statements and Guidelines should be directed to the AHA/ASA Manuscript Oversight Committee via its Correspondence page.Sign In to Submit a Response to This Article Previous Back to top Next FiguresReferencesRelatedDetailsCited By Khalaf F, Dube P, Mohamed A, Tian J, Malhotra D, Haller S and Kennedy D (2018) Cardiotonic Steroids and the Sodium Trade Balance: New Insights into Trade-Off Mechanisms Mediated by the Na+/K+-ATPase, International Journal of Molecular Sciences, 10.3390/ijms19092576, 19:9, (2576) Touza N, Pôças E, Quintas L, Cunha-Filho G, Santos M and Noël F (2011) Inhibitory effect of combinations of digoxin and endogenous cardiotonic steroids on Na+/K+-ATPase activity in human kidney membrane preparation, Life Sciences, 10.1016/j.lfs.2010.10.027, 88:1-2, (39-42), Online publication date: 1-Jan-2011. Jaques-Robinson K, Golfetti R, Baliga S, Hadzimichalis N and Merrill G (2008) Acetaminophen Is Cardioprotective Against H 2 O 2 -Induced Injury In Vivo , Experimental Biology and Medicine, 10.3181/0802-RM-68, 233:10, (1315-1322), Online publication date: 1-Oct-2008. Schoner W and Scheiner-Bobis G (2007) Endogenous and exogenous cardiac glycosides: their roles in hypertension, salt metabolism, and cell growth, American Journal of Physiology-Cell Physiology, 10.1152/ajpcell.00098.2007, 293:2, (C509-C536), Online publication date: 1-Aug-2007. Schoner W and Scheiner-Bobis G (2007) Endogenous and Exogenous Cardiac Glycosides and their Mechanisms of Action, American Journal of Cardiovascular Drugs, 10.2165/00129784-200707030-00004, 7:3, (173-189), . March 2006Vol 47, Issue 3 Advertisement Article InformationMetrics https://doi.org/10.1161/01.HYP.0000202641.29167.c0PMID: 16461849 Originally publishedFebruary 6, 2006 PDF download Advertisement