Title: Cofactor requirements of microsomal 3β-hydroxy-steroid oxidoreductase, 5-ene-isomerase from human placenta
Abstract: Cofactor requirements of 3β-hydroxysteroid oxidoreductase, 5-ene-isomerase were studied in lyophilized microsomes and in supernatants obtained by washings with buffer solution. Following 1 h incubation with lyophilized microsomes the activity with NAD or NADP was almost equal. However, rate measurements using 1 or 5 min incubations showed that NAD was the most active cofactor. In 120,000 g supernatants of three successive washings the activity was mainly NAD dependent. The possibility that the activity found in the presence of NADP is mediated by a microsomal transhydrogenase is discussed.
Publication Year: 1975
Publication Date: 1975-08-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 5
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