Title: Functional bacteriochlorophyll-protein complexes from chromatophores of Rhodopseudomonas spheroides strain R-26
Abstract: 1. The chromatophore membrane system of the blue-green mutant strain R-26 of Rhodopseudomonas spheroides has been fractionated by Trition X-100 into two separable and distinct kinds of particles, each containing one of the two functional forms of bacteriochlorophyll. The smaller reaction center particles contain only the reaction center bacteriochlorophyll, P870, and the associated P800. The relative ratios of electron transfer components in these preparations are about the same as in chromatophores. The reaction centers contain 4.4·105 g of protein per mole of P870 and appear predominantly as small particles in electron micrographs. 2. By contrast, the particles of the light-harvesting component contain the light-harvesting bacteriochlorophyll and are nearly devoid of reaction centers. Micrographs show particles as large smooth discs. The preparations contain 2.5·104 g of protein per mole of bacteriochlorophyll. These large light-harvesting component particles are further fractionated by sodium dodecyl sulfate into sodium dodecyl sulfate fragments, small bacteriochlorophyll proteins. The chemical and physical properties of these subchromatophore particles are discussed with special regard to the organization of the chromatophore membrane.
Publication Year: 1970
Publication Date: 1970-12-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 22
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot