Title: Carboxymethylated liver alcohol dehydrogenase: pH dependence of hydride transfer during ethanol oxidation
Abstract: The rate constant for the hydride transfer step during oxidation of ethanol by cabroxymethylated alcohol dehydrogenase (alcohol:NAD+ oxidoreductase, EC 1.1.1.1) is dependent on a group with pKa 7.5. This pKa is higher than that for the native enzyme. A mechanism is proposed to account for the ionisation and the protein fluorescence change which occur during formation of the carboxymethylated enzyme-NAD+-ethanol complex.
Publication Year: 1985
Publication Date: 1985-10-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 2
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