Title: Inactivation of cultured human cells and control of C3H mouse mammary tumors with accelerated nitrogen ions
Abstract: CancerVolume 32, Issue 3 p. 541-546 ArticleFree Access Inactivation of cultured human cells and control of C3H mouse mammary tumors with accelerated nitrogen ions Darrell Q. Brown PHD, Darrell Q. Brown PHD Department of Radiation Therapy, The American Oncologic Hospital, Philadelphia, Pa.; The Institute for Cancer Research, Philadelphia; The Pennsylvania State University, University Park, Pennsylvania; and The Princeton Particle Accelerator, Princeton, N.J. Assistant Professor of Radiology, University of Pennsylvania School of Medicine.Search for more papers by this authorH. Gunter Seydel MS, MD, H. Gunter Seydel MS, MD Department of Radiation Therapy, The American Oncologic Hospital, Philadelphia, Pa.; The Institute for Cancer Research, Philadelphia; The Pennsylvania State University, University Park, Pennsylvania; and The Princeton Particle Accelerator, Princeton, N.J. Associate Professor of Radiology, University of Pennsylvania School of Medicine.Search for more papers by this authorPaul Todd PHD, Paul Todd PHD Department of Radiation Therapy, The American Oncologic Hospital, Philadelphia, Pa.; The Institute for Cancer Research, Philadelphia; The Pennsylvania State University, University Park, Pennsylvania; and The Princeton Particle Accelerator, Princeton, N.J. Associate Professor of Biophysics, Pennsylvania State University.Search for more papers by this author Darrell Q. Brown PHD, Darrell Q. Brown PHD Department of Radiation Therapy, The American Oncologic Hospital, Philadelphia, Pa.; The Institute for Cancer Research, Philadelphia; The Pennsylvania State University, University Park, Pennsylvania; and The Princeton Particle Accelerator, Princeton, N.J. Assistant Professor of Radiology, University of Pennsylvania School of Medicine.Search for more papers by this authorH. Gunter Seydel MS, MD, H. Gunter Seydel MS, MD Department of Radiation Therapy, The American Oncologic Hospital, Philadelphia, Pa.; The Institute for Cancer Research, Philadelphia; The Pennsylvania State University, University Park, Pennsylvania; and The Princeton Particle Accelerator, Princeton, N.J. Associate Professor of Radiology, University of Pennsylvania School of Medicine.Search for more papers by this authorPaul Todd PHD, Paul Todd PHD Department of Radiation Therapy, The American Oncologic Hospital, Philadelphia, Pa.; The Institute for Cancer Research, Philadelphia; The Pennsylvania State University, University Park, Pennsylvania; and The Princeton Particle Accelerator, Princeton, N.J. Associate Professor of Biophysics, Pennsylvania State University.Search for more papers by this author First published: September 1973 https://doi.org/10.1002/1097-0142(197309)32:3<541::AID-CNCR2820320305>3.0.CO;2-PCitations: 6 AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Accelerated nitrogen ions (N7+) from the Princeton Particle Accelerator were used to irradiate three dimensional cultures of human kidney T-l cells and 2 mm thick transplanted C3H mouse mammary tumors. Cell survival was determined at the Bragg peak and at the entrance portion (“plateau”) of the beam using colony formation as the survival criterion. Resulting cell survival curves are compared with those for x-irradiation under hypoxic and aerobic conditions. The mammary tumors were given single N7+ fractions of 690, 1400, 2800, and 5500 rads in the Bragg peak. The resulting tumor cure dose for 50% of the animals (TCD50.,−120) was determined to be 2400 rads as compared with 5100 rads with x rays. The relative biological effectiveness (RBE) decreased with increasing dose in both systems and ranged between 1.5 and 4.5 for aerobic human cells, 3.7 and 12 for hypoxic human cells, 0.96 and 1.6 for acute mouse skin reaction, 1.9 and 2.5 for mouse tumor control. The RBE for tumor control was consistent with that for hypoxic cells. These results appear to support the proposed use of heavy ions in radiotherapy, at least in cases of small, localized tumors, although further study is needed to determine their effectiveness in the treatment of large volumes, which would require irradiation with multiple Bragg peaks. References 1 Barendsen, G. W.: Cellular responses determining the effectiveness of fast neutrons relative to x rays for effects on experimental tumors. Europ. J. Cancer 7: 181– 190, 1971. 2 D'Angio, G. J., Lawrence, J. H., Gottschalk, A., and Lyman, J.: Relative efficiency of high-LET radiation (Bragg-peak Lithium ions) on normal rabbit skin, using integral dose as a basis for comparison. Nature 204: 1267– 1268 1964 3 Field, S. B., and Hornsey, S.: RBE values for cyclotron neutrons for effects on normal tissues and tumors as a function of dose and dose fractionation. Europ. J. Cancer 7: 161– 169 1971 4 Finney, D. J.: Probit Analysis, 2nd ed. Cambridge, Cambridge University Press, 1962. 5 Fry, R. J. M., Gregg, E. C., Painter, R. B., and Roesch, W. C.: High-LET radiation in radiotherapy. Radiology 103: 215– 220 1972 6 Gosalvez, M., Thurman, R. G., Chance, B., and Reinhold, H.: Regional variation in the oxygenation of mouse mammary tumors in vivo demonstrated by fluorescence of pyridine nucleotide. Br. J. Radiol. 45: 510– 514 1972 7 Hewitt, H. B., and Wilson, C. W.: Survival curves for tumor cells irridated in vivo. Ann. N. Y. Acad. Sci. 95: 818– 827 1961 8 Kellerer, A. M., and Rossi, H. H.: RBE and the primary mechanism of radiation action. Radiat. Res. 47: 15– 34 1971 9 Schimmerling, W., Vosburgh, K. G., and Todd, P.: Personal communication, 1972. 10 Suit, H. D., 1969 (See Bond, V. P.: Negative pions: Their possible use in radiotherapy. Am. J. Roentgenol. 111: 9– 26, 1971). 11 Suit, H. D., and Maeda, M.: Hyperbaric oxygen and radiobiology of a C3H mouse mammary carcinoma. J. Natl. Cancer Inst. 39: 639– 652 1967 12 Thomlinson, R. H., and Gray, L. H.: The histological structure of some human lung cancers and the possible implications for radiotherapy. Br. J. Cancer 9: 539– 559 1955 13 Tobias, C. A., and Todd, P. W.: Heavy charged particles in cancer therapy. Natl. Cancer Inst. Monogr. 24, U. S. Department of Health, Education, and Welfare, National Cancer Institute, Bethesda, Md., 1967 pp. 1– 21 14 Todd, P.: Heavy-ion irradiation of cultured human cells. Radiat. Res. Suppl. 7: 196– 207 1967 15 Todd, P.: Fractionated heavy ion irradiation of cultured human cells. Radiat. Res. 34: 378– 389 1968 16 Urbach, F., and Noell, W. K.: Studies on the oxygen supply of tumor tissue. Proc. Am. Assoc. Cancer Res. 2: 257A, 1957. 17 White, M. G., Isaila, M., Prelec, K., and Allen, H. L.: Acceleration of nitrogen ions to 7.4 GeV in the Princeton Particle Accelerator. Science 174: 1121– 1123 1971 Citing Literature Volume32, Issue3September 1973Pages 541-546 ReferencesRelatedInformation