Title: Site-Directed Spin Labeling and Electron Paramagnetic Resonance (EPR) Spectroscopy: A Versatile Tool to Study Protein-Protein Interactions
Abstract: Protein Interactions 428 about almost all aspects of this interaction.Spin labeling approaches can provide detailed information about the binding interface not only on the structural level but also give insights into kinetic and thermodynamic aspects of the interaction.EPR also allows determination of distances between pairs of spin labels in the range from ~ 10-80 Å with accuracies down to less than 1 Å, thereby covering a range of sizes including also large multi-domain proteins and protein complexes.This chapter will give an introduction into the technique of SDSL EPR spectroscopy exemplified with data from studies on the photoreceptor/transducer-complex NpSRII/NpH t r I I , f o l l o w e d b y a n u m b e r o f r e c e n t examples from the literature where protein-protein interactions have been studied using this technique.