Title: Exploiting Somaclonal Variation — Especially GENE Introgression from Alien Chromosomes
Abstract: The following example is discussed of using somaclonal variation to achieve transfer of valuable genes from an alien chromosome to a crop plant. Barley yellow dwarf virus (BYDV) resistance has been transferred to wheat (Triticum aestivum) from a disomic addition line with a Thinopyrum (Agropyron) intermedium chromosome. The resistance locus is on the long arm of the 7Ai-1 chromosome. BYDV resistant recombinant lines were identified after selection against a short arm marker (red coleoptile) and for BYDV resistance. Resistance in seven of the cell culture induced recombinants has been inherited with Mendelian segregation ratios for eight generations. Heterozygote meiosis indicates that the alien chromatin in the cell culture induced recombinants is small enough to allow regular meiotic behaviour. A probe, pEleAcc2, hybridising to dispersed repetitive sequences, was used to detect Th. intermedium chromatin. The translocations involved a portion of alien chromatin less than the long arm of 7Ai-1. RFLP analysis confirmed the loss of a short arm marker (probe psrl52) but the retention of two long arm markers (probes psr129 and csIH81-l) which assort with the BYDV resistance. A third long arm alien marker (probe psr690), was absent in one of the recombinants.
Publication Year: 1995
Publication Date: 1995-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
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Cited By Count: 1
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